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【摘要】 目的 研究缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)、微血管密度(MVD)在腺样囊性癌(ACC)中的表达和相关性,并探讨它们在腺样囊性癌侵袭和转移生物学行为的机理。方法 应用免疫组化SP法,检测35例腺样囊性癌、13例多形性腺瘤和10例正常唾液腺组织中HIF-1α、VEGF的表达和MVD计数。 结果 HIF-1α、VEGF、MVD计数在正常唾液腺组织、多形性腺瘤、ACC中依次增加,组间差异具有统计学意义(P 1.3 方法 HIF-1α、VEGF和CD34的检测采用SP免疫组化法,染色程序按S-P试剂盒说明书进行。用PBS代替一抗作阴性对照,用已知阳性表达组织(乳腺癌)作阳性对照。
1.4结果判断
1.4.1HIF-1α、VEGF着色所有阳性表达均为棕黄色,定位于细胞浆或细胞核,以阳性细胞数量作为观察指标,至少观察10个高倍视野中阳性肿瘤细胞数占所观察肿瘤细胞总数的百分比,50%记为“++”[3]。
1.4.2 MVD的测定 根据Weidner[4]法进行MVD计数:先在低倍镜下扫视整个切片区寻找微血管高密度区即热点,然后在200倍光镜下计数被染成棕色的血管内皮细胞簇,结果用3个200倍视野下血管数目的平均数来表示。
表1
HIF-1α蛋白在正常唾液腺组织、多形性腺瘤、腺样囊性癌中的表达
分组例数
HIF-1α表达
-+++
正常唾液腺组织1010(100.00%)0(0.00%)0(0.00%)
多形性腺瘤1311(84.70%)2(15.30%)0(7.65%)
腺样囊性癌3510(28.60%)15(42.80%)10(28.60%)
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