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[摘要] 目的:观察不同类型男性冠心病患者血清睾酮、游离睾酮与血管紧张素-Ⅱ(angiotensin Ⅱ, AngⅡ)水平改变及相互间关系,进一步探讨睾酮与AngⅡ在男性急性冠脉综合征(acute coronary syndrome,ACS)发病中的作用。方法:冠心病(coronary heart disease,CHD)患者分为急性心肌梗死(acute myocardial infarction,AMI)组、不稳定型心绞痛(unstable angina pectoris,UAP)组、稳定型心绞痛(stable angina pectoris,SAP)组,每组30例患者,另设健康对照组30例,比较各组间血清睾酮、游离睾酮与AngⅡ水平差异并分析其相关性。结果:AMI组和UAP组血清游离睾酮与AngⅡ水平与对照组比较,差异有统计学意义(P 1.2 研究方法
1.2.1 标本的收集各组均于入院后未给予任何处置和治疗前抽取静脉血5 ml,静置待血液凝固后离心分离出血清,-70℃冰箱冷冻保存,统一测定。
1.2.2 血清睾酮、游离睾酮的检测方法检测方法均采用酶联免疫吸附法(enzyme-linked emmunosorbent assay,ELISA)测定,试剂盒由 Sigma公司提供,测定步骤严格按照试剂盒说明书进行。
1.2.3 AngⅡ的检测方法采用放射免疫法(radioemmuno assay,REA)测定,试剂盒由Sigma公司提供,测定步骤严格按照试剂盒说明书进行。
1.3 统计学处理
所有计量资料以x±s表示,采用统计软件SPSS 13.0对结果进行分析,两样本均数比较采用t检验,各变量相互关系采用Logistic回归分析和直线相关分析,正态性检验采用D检验,相关系数的统计意义检验采用t检验,P0.05,相关系数相比,差异无统计学意义。
2.6 ACS患者总胆固醇水平和AngⅡ水平的相关性
AMI组和UAP组合并后(n=60)总胆固醇水平和AngⅡ水平的相关分析,r=0.231 6,tr=1.209 4,t0.05(58)=2.000,tr0.05,相关系数相比,差异无统计学意义。
2.7 ACS患者血清游离睾酮和AngⅡ水平的正态性检验
AMI组和UAP组合并后(n=60)血清游离睾酮和AngⅡ水平的正态性分析,n=60,y0.05(60)= -2.17,D血清游离睾酮=0.300 4,y血清游离睾酮=-1.098 3,y血清游离睾酮>y0.05(60),D AngⅡ=0.321 9,yAngⅡ=-1.037 2,yAngⅡ>y0.05(60),P
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(收稿日期:2009-06-08)
