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[摘要] 目的:研究荧光原位杂交技术在检测宫颈脱落细胞中hTERC基因扩增情况,为女性子宫颈病变的筛查、早期诊断及预后提供新途径。方法:收集60例妇女患者进行宫颈脱落细胞的液基细胞学检查(TCT)、第2代杂交捕获法(HC2)高危型人乳头瘤病毒(HPV)检测(HPV-DNA)、FISH法hTERC基因检测及经阴道镜下宫颈活检病理证实,做出宫颈病变的最后诊断。结果:随着细胞学病变程度的加重及宫颈上皮内瘤变(CIN)级别增加,hTERC基因扩增阳性率增加(χ2=11.111,χ2=10.824,P 1.3 FISH判断标准
应用hTERC基因双色探针(hTERC探针购置于北京金菩嘉医疗科技有限公司,为TERC/CSP3DNA探针),HPV阴性、病理诊断为炎症的宫颈脱落上皮中hTERC基因杂交信号为标准确定。取涂片,分别计数100个细胞,记录hTERC基因超过2个杂交信号的异常细胞数(即细胞中出现绿:红 [6] Andersson S, Sokolova I, Algeciras-Schimnich A,et al.Chromosomal biomarkers for detection of human papillomavirus associated genomic instability in epithelial cells of cervical cytology specimens[J]. J Mol Diagn,2007,9(5):604-611.
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(收稿日期:2010-04-09)
