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[摘要] 目的:探讨自组装纤维支架肽RADA16在皮肤烫伤中的治疗作用。方法:在SD大鼠背部以电力机械方法制造深Ⅱ度烧伤模型,对照治疗组以0.9%NaCl溶液代替,烧伤后按时换药,并在不同时间段(伤后7、10、14 d分别取烧伤修复创面的皮肤组织进行免疫组化染色,记录创面碱性成纤维细胞生长因子(bFGF)及表皮细胞生长因子(EGF)的表达,并以图像处理系统软件记录生长因子表达的半定量检测。比较了短肽处理组及空白对照组创面生长因子表达的灰度值。结果:免疫组织化学结果显示,在烧伤修复的不同修复期,短肽处理组bFGF和EGF均明显表达在新生表皮组织中,与空白对照组比较,差异均有统计学意义(P 1.4统计学分析
采用SPPS 13.0统计软件进行分析,组间比较采用单因素方差分析(ANOVA),正态分布式数据以Turkey分析, 非正态分布数据采用posthoc分析。
2结果
为了进一步探讨自组装短肽治疗皮肤创伤组织的可能机制,以链霉素抗生物素过氧化物酶免疫组织化学研究再上皮化的烧伤皮肤组织中是碱性成纤维细胞生长因子(bFGF)和表皮生长因子(EGF)的表达。实验组及对照组处理伤口的bFGF和EGF的表达及分布见表1。在伤后第7天及第10天,肽治疗组,bFGF在表皮和腺体明显表达。bFGF的表达的增强(P99.5%(M/V))形成高度交织纳米纤维支架凝胶。形成天然的三维细胞外基质支架适合细胞迁移,诱导和调节各种细胞因子和生长。当这种生物敷料被倍比稀释,其形成的自组装纤维从球状聚集(4.8±0.38) nm的高度,宽度(61.6±6.10) nm,到(708±80.2) nm的长度,仍维持明显的纳米纤维结构,有利于细胞的爬行及生长。
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(收稿日期:2010-05-10)
