【www.zhangdahai.com--教师述职报告】
[摘要] 目的:探讨宫颈鳞癌及癌前病变组织中整合素连接激酶(ILK)、PTEN的表达情况。方法:采用免疫组化方法对50例宫颈鳞癌,28例CIN和12例正常宫颈组织进行ILK、PTEN检测,并结合临床资料进行统计分析。结果:宫颈鳞癌ILK阳性表达率明显高于正常组织和CIN 1、2级组织,ILK表达与肿瘤浸润、转移和临床分期密切相关;正常宫颈组织PTEN均呈阳性表达,而宫颈浸润癌组织PTEN表达率明显降低,但PTEN表达与肿瘤浸润、转移和临床分期无明显相关性。结论:宫颈鳞癌组织ILK呈高表达、PTEN呈低表达,可能在宫颈肿瘤浸润转移过程中起重要作用。
[关键词] 宫颈鳞癌;整合素连接激酶;免疫组织化学
[中图分类号] R737.33 [文献标识码]A [文章编号]1674-4721(2010)04(a)-05-03
The expression of ILK,PTEN in cervical squamous carcinoma and cervical precancerous lesions
LIU Chunzai1, LI Chunling2, LI Shengshui3*, JIANG Ze4, LI Shuangbiao3, XU Hua3, ZHANG Fengmei3, LIU Yan3
(1.Nan Da Gang Hospital, Cangzhou061103, China; 2.Cangzhou Women & Children’s healthcare Hospital, Cangzhou061001, China; 3.Cangzhou Hospital for Integrated Chinese and Western Medicine, Cangzhou061001, China; 4.Cangzhou Central Hospital, Cangzhou061001, China)
[Abstract] Objective: To study the expression of ILK,PTEN in cervical squamous carcinoma and cervical precancerous lesions. Methods:50 cases of cervical squamous carcinoma, 28 cases of cervical intraepithelial neoplasia( CIN) and 12 cases of normal cervical tissues ample were stained for ILK,PTEN by immunohistochemistry method. Results: ILK positive expression rate in cervical squamous carcinoma was significantly higher than that in normal cervical tissue and CIN I. The positive expression rate of PTEN in normal cervical tissue was 100.0%, but it was remarkably decreased in cervical squamous carcinoma. Our analysis indicated that no significant relationship was found between the state of PTEN expression and invasion,metastasis and the clinical stage of the tumor. Conclusion: The higher expression of ILK and the lower expression of PTEN in cervical squamous carcinoma may play an important role in angiogenesis of cervical squamous carcinoma.
[Key words] Cervical squamous carcinoma; ILK; Immunohistochemistry
宫颈鳞状细胞癌(cervical squamous cell carcinoma, SCC)是世界上发病率仅次于乳腺癌的女性第二常见肿瘤,目前仍是女性生殖系统发病率最高的恶性肿瘤,且近年发病率趋于年轻化,严重危害女性生理、心理健康[1]。近年来,随着分子生物学的发展,人们开始关注SCC发生、发展的分子生物学机制。本文应用免疫组化方法对宫颈SCC进行整合素连接激酶(ILK)、PTEN检测,探讨它们在宫颈SCC中表达及临床意义。
1 材料与方法
1.1 标本来源
选取5家医院2005～2009年手术及活检宫颈标本90例。正常宫颈组织12例(子宫肌瘤行全子宫切除术),CIN 28例,宫颈浸润性鳞状细胞癌50例, 10%中性甲醛液固定,均由病理专家确诊。宫颈浸润性鳞状细胞癌按WHO分级标准分为高分化(G1)、中分化(G2)和低分化(G3),按1995年FIGO标准分为Ⅰ、Ⅱ、Ⅲ、Ⅳ期。
1.2 主要试剂与方法
鼠抗人ILK、PTEN及免疫组化试剂盒购自北京中杉生物技术有限公司。免疫组化采用S-P法,按照试剂盒操作步骤进行,DAB显色,苏木精复染,中性树胶封固。
1.3 结果判定
ILK、PTEN均主要为胞质着色,以胞质内出现棕黄色颗粒的细胞为阳性染色细胞,每例均随机观察5个高倍视野(×400),用半定量积分法判断结果,阳性细胞率5%为0分,6%～25%为1分,26%～50%为2分,51%～75% 为3分,>75 %为4分;阳性强度黄色为1分,棕黄为2分,棕褐色为3分。两者积分相乘,0分为阴性(-),1～4分为弱阳性(+),5～8分为中度阳性(++),9～12分为强阳性(+++)。
1.4 统计学分析
采用t检验和χ2检验。
2 结果
2.1 宫颈鳞癌及CIN中ILK的表达情况
正常宫颈组织和CIN I组织中几乎不表达ILK,CINⅡ中阳性表达率也很低,与CIN Ⅲ和浸润性宫颈鳞癌相比,差异有统计学意义 (P 2.4 ILK、PTEN表达宫颈鳞癌临床病理特征的关系
宫颈浸润性鳞状细胞癌组ILK阳性表达率随着组织学分级的升高而增高,有淋巴结转移者明显高于无淋巴结转移者,浸润深度≥1 cm者明显高于浸润深度
